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为了研究纳米颗粒与蛋白质的相互作用原理,我们选取纳米金颗粒及牛血清蛋白为材料,通过牛血清蛋白与纳米金颗粒的物理吸附作用,结合蛋白酶K酶切、SDS洗脱以及SDS-聚丙烯酰胺凝胶电泳检测的方法,我们了解到,纳米颗粒与蛋白质的结合主要是由蛋白质中的某一段特定的序列结合在纳米颗粒的表面所导致的,该结果的发现不仅对纳米颗粒在生物体内广泛的应用奠定了理论基础,对功能性纳米颗粒在生物体内功能的发挥也是至关重要的。
In order to study the principle of the interaction between nanoparticles and protein, we selected nano-gold particles and bovine serum albumin as material, through the physical adsorption of bovine serum albumin and nano gold particles, combined with protease K digestion, SDS elution and SDS-polypropylene Amide gel electrophoresis test method, we understand that the combination of nanoparticles and protein is mainly due to a particular sequence of protein binding to the surface of the nanoparticles caused by the discovery of this result not only for the nanoparticles in the body It has laid a theoretical foundation for a wide range of applications and is also of crucial importance for the functional development of functional nanoparticles in vivo.