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目的:探讨siRNA(small interferingRNA,siRNA)干扰β-catenin对人胶质瘤细胞系SHG44体外增殖和凋亡的影响。方法:利用脂质体将β-catenin siRNA干扰片段转染SHG44细胞,免疫印迹和免疫荧光共聚焦技术观察β-catenin表达及分布的变化;通过MTT法观察细胞增殖的变化;集落形成试验观察细胞增殖能力的改变;Annexin V/PI双染流式细胞术观察细胞凋亡情况。结果:β-catenin siRNA转染SHG44细胞可以有效抑制β-catenin的表达,并抑制其向核内的转移和聚集。与对照组相比,β-catenin siRNA转染组能够抑制细胞增殖,抑制细胞的集落形成能力,增加了细胞的凋亡,且均具有统计学意义,P<0.01。结论:通过β-catenin siRNA抑制SHG44细胞β-catenin的表达,可以抑制细胞增殖,促进其凋亡,表明β-catenin在胶质瘤的发生发展中发挥重要作用。
AIM: To investigate the effect of siRNA on the proliferation and apoptosis of human glioma cell line SHG44 in vitro. Methods: The β-catenin siRNA transfection was transfected into SHG44 cells by lipofectamine. The expression of β-catenin and its distribution were observed by Western blotting and immunofluorescence confocal microscopy. The cell proliferation was observed by MTT assay. Proliferation and proliferation. Annexin V / PI double staining flow cytometry was used to observe the cell apoptosis. Results: Transfection of β-catenin siRNA into SHG44 cells could effectively inhibit the expression of β-catenin and inhibit its migration to the nucleus. Compared with the control group, β-catenin siRNA transfection group could inhibit cell proliferation, inhibit cell colony forming ability and increase cell apoptosis, all of which had statistical significance (P <0.01). Conclusion: The inhibitory effect of β-catenin siRNA on the expression of β-catenin in SHG44 cells can inhibit cell proliferation and promote apoptosis, indicating that β-catenin plays an important role in the development and progression of glioma.