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目的观察 TGF—β_1在阻塞性黄疸肝组织中的表达,并初步探讨其作用。方法运用Wistar 大鼠建立阻塞性黄疸模型,观察肝脏病理变化及血清胆红素的变化;运用免疫组织化学方法检测肝脏组织中 TGF—β_1的表达;并运用原位末端标记方法检测肝细胞凋亡。结果:Wistar 大鼠结扎胆总管后,胆红素急剧升高,术后1周可见肝脏表达 TGF—β_1,肝细胞出现大量的凋亡,纤维组织少量增生。术后2周,TGF—β_1表达及细胞凋亡达到高峰,纤维组织大量增生。术后3周,纤维组织占据肝脏组织大部分,细胞凋亡相对减少,仍可见大量的 TGF—β_1表达。结论在阻塞性黄疸中,肝脏表达大量的 TGF—β_1,与肝细胞凋亡、纤维化及机体免疫功能抑制密切相关。
Objective To observe the expression of TGF-β 1 in the liver of obstructive jaundice and to explore its role. Methods Wistar rats were used to establish the model of obstructive jaundice. The pathological changes of liver and the changes of serum bilirubin were observed. The expression of TGF-β 1 in liver tissue was detected by immunohistochemical method. The apoptosis of hepatocytes was detected by in situ end labeling . Results: The bilirubin increased sharply after ligating the common bile duct in Wistar rats. One week after operation, TGF-β1 was expressed in the liver, and a large number of apoptosis occurred in the hepatocytes and a small amount of fibrous tissue proliferated. After 2 weeks, the expression of TGF-β 1 and apoptosis peaked, and the fibrous tissue proliferated. At 3 weeks after operation, most of the liver tissue was occupied by fibrous tissue, and the apoptosis rate was relatively decreased. However, a large number of TGF-β 1 was still found. Conclusions In obstructive jaundice, the liver expresses a large number of TGF-β 1, which is closely related to the inhibition of hepatocyte apoptosis, fibrosis and immune function.