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The present short review aims to give an overview of the most recent developments in fluorescence microscopy and its applications in biomedical sciences. Apart from improvements in well established methods based on conventional fluorescence microscopy and confocal microscopy (fluorescence in situ hybridisation (FISH), tyramide signal amplification (TSA) in immunocytochemistry, new fluorophores), more recently introduced techniques like fluorescence resonance energy transfer (FRET), fluorescence recovery after photobleaching (FRAP), multiphoton microscopy and fluorescence correlation spectroscopy (FCS) will be discussed.
The present short review aims to give an overview of the most recent developments in fluorescence microscopy and its applications in biomedical sciences. Apart from improvements in well established methods based on conventional fluorescence microscopy and confocal microscopy (fluorescence in situ hybridization (FISH) amplification (TSA) in immunocytochemistry, new fluorophores), more recently introduced techniques like fluorescence resonance energy transfer (FRET), fluorescence recovery after photobleaching (FRAP), multiphoton microscopy and fluorescence correlation spectroscopy (FCS) will be discussed.