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背景:实验小组前期研究发现孕鼠宫内缺氧可刺激胎鼠神经干细胞的增殖,缺氧6h时增殖达高峰,在9h也表现增殖,但能力开始下降。而缺氧达12h时即表现为坏死或凋亡,但随缺氧天数的延长及时段的不同,对神经干细胞的影响又如何?目的:进一步探讨宫内缺氧对新生大鼠神经干细胞增殖、分化的影响及当归注射液的保护作用。方法:孕SD大鼠随机分为对照组、缺氧组和当归治疗组。孕14d开始将当归组与缺氧组孕鼠置于三气培养箱中,制作缺氧性脑损伤新生鼠模型,此前1h分别给于当归注射液和生理盐水尾静脉注射,对照组不缺氧,余同缺氧组。孕鼠分娩后立即取新生鼠大脑组织,经胶质纤维酸性蛋白、神经元特异性烯醇化酶免疫组织化学染色后行图像分析。结果与结论:①缺氧组新生鼠海马胶质纤维酸性蛋白免疫组织化学阳性细胞的表达较相应对照组增加;而神经元特异性烯醇化酶免疫组织化学阳性细胞的表达较对照组减小。②当归治疗组新生鼠海马胶质纤维酸性蛋白免疫组织化学阳性细胞的表达较相应缺氧组减少;而神经元特异性烯醇化酶免疫组织化学阳性细胞的表达较对照组增大。结果表明,一定程度的缺氧可刺激神经干细胞增殖,并可刺激神经干细胞向神经胶质细胞分化,以及导致神经元的减少;当归注射液可减弱由于缺氧导致的神经干细胞的增殖和向胶质细胞分化的能力,并可缓解神经元的减少,提示当归可能对缺氧大鼠神经系统有一定的保护作用。
BACKGROUND: The experimental group found that intrauterine hypoxia could stimulate the proliferation of neural stem cells in fetal rat embryos. The peak of proliferation reached the peak at 6h of hypoxia and also proliferated at 9h, but its ability began to decline. However, hypoxia up to 12h showed necrosis or apoptosis, but with the hypoxia days and different time periods, the impact of neural stem cells and how? Objective: To explore the intrauterine hypoxia on the proliferation of neural stem cells in neonatal rats, Differentiation and the Protective Effect of Danggui Injection. Methods: Pregnant SD rats were randomly divided into control group, hypoxia group and Angelica treatment group. Pregnant 14d began to Angelica group and hypoxic group of pregnant rats placed in three gas incubator, hypoxic brain injury in neonatal rat model, after 1h were given Angelica injection and saline intravenous injection, the control group without hypoxia , With the same hypoxia group. The neonatal rat brain tissue was taken immediately after delivery of the pregnant mice, and then analyzed by glial fibrillary acidic protein and neuron specific enolase immunohistochemical staining. RESULTS AND CONCLUSION: ① The expression of hippocampal glial fibrillary acidic protein immunocytochemical positive cells in hypoxic group increased compared with the corresponding control group, while the expression of neuron specific enolase immunohistochemical positive cells decreased compared with the control group. ② The expression of glial fibrillary acidic protein immunocytochemical positive cells in the hippocampus of Angelica sinensis treatment group was lower than that in the corresponding hypoxia group, while the expression of neuron specific enolase immunohistochemical positive cells increased compared with the control group. The results showed that a certain degree of hypoxia stimulated the proliferation of neural stem cells and stimulated the differentiation of neural stem cells into glial cells and resulted in the decrease of neurons. Angelica injection could attenuate the proliferation of neural stem cells induced by hypoxia, The ability of differentiation of neurons and mitigation of neurons, suggesting that Angelica may have a protective effect on the nervous system of hypoxic rats.