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目的了解10年前我国HBsAg阳性献血员中HBV，HCV，HGV重叠感染状况。方法采用逆转录套式PCR技术检测血清中HCWI：NA、HGVRNA，免疫PCR技术检测HBVDNA。结果发现在HBsAg阳性献血员中，HBVDNA阳性率90％，HCVRNA阳性率20％，HGVRNA阳性率6％，三重感染阳性率3％。结论HBsAg阳性献血员中有较高比例的双重感染，且以HCV感染为主。
Objective To understand the overlapping infection status of HBV, HCV and HGV in HBsAg positive blood donors in China 10 years ago. Methods Serum HCWI: NA and HGVRNA were detected by reverse transcription polymerase chain reaction (RT-PCR), and HBVDNA was detected by immune PCR. The results showed that in HBsAg positive blood donors, HBVDNA positive rate of 90%, HCVRNA positive rate of 20%, HGVRNA positive rate of 6%, triple infection rate of 3%. Conclusion HBsAg positive blood donors have a higher proportion of double infection, and mainly HCV infection.