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[目的 ]观察日本血吸虫病流行区人群的细胞免疫应答状态及吡喹酮治疗对其影响。 [方法 ]采集日本血吸虫病流行区 12 9人 (粪检阳性者 6 4例 ,粪检阴性者 6 5例 ) ,吡喹酮治疗前及治疗后 45d的静脉血 ,以血吸虫成虫和虫卵抗原分别刺激诱生细胞因子 ,检测培养上清中的细胞因子IL 5、IL 10和IFN γ水平。 [结果 ]12 9例中 ,对血吸虫抗原刺激诱生的细胞因子水平粪检阴性组明显高于粪检阳性组 ;治疗后 ,细胞因子诱生水平较治疗前显著升高 ,特别是IL 5和IFN γ。 [结论 ]流行区人群的细胞免疫应答显示总体下调趋势 ;吡喹酮治疗后出现细胞因子水平的明显升高。
[Objective] To observe the state of cellular immune response in schistosomiasis japonica population and the effect of praziquantel treatment on it. [Methods] A total of 129 schistosomiasis japonica endemic areas were collected, including 64 positive stool tests and 65 negative stool tests, venous blood before and after 45 days of praziquantel treatment, The induced cytokines were respectively stimulated and the levels of cytokines IL 5, IL 10 and IFN γ were detected in the culture supernatant. [Results] In 12 9 cases, the levels of cytokines induced by schistosome antigen stimulation were significantly higher in the fecal negative group than those in the fecal positive group. After treatment, the levels of cytokine induced were significantly higher than those before treatment, especially IL 5 and IFN γ. [Conclusion] The cellular immune responses in epidemic areas showed an overall downward trend; the levels of cytokines were significantly increased after praziquantel treatment.