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从辽宁分离的快生型大豆根瘤菌在柠檬酸盐培养基上表现出两种类型。12株中7株在柠檬酸盐培养基上不生长,5株生长良好。但生长速度比A.tumefaciens慢些。所有菌株都不产生3-酮基乳糖。在石蕊牛奶上除1株以外,都产生血清环,3株产酸,8株产碱1株不明显。在耐盐方面一般能耐0.3 M—0.4MNaCl,而能利用柠檬酸盐的菌株有4株能耐0.45 M Nacl的浓度。这些菌株和我国的栽培大豆品种铁丰18、开育8号、77—153等品种都能进行有效共生。但在干重、植株含氮量上均显著低于慢生型大豆根瘤菌。快型菌株和铁丰18、开育8号亲和性较好、77—153较差。111、121、123 A分别接在豇豆(北京红)和绿豆(D0245—1)、上,除111外其余两株都结瘤并有固氮酶活性。所有结瘤试验都用相应抗血清做了鉴定,证明确系由快生型大豆根瘤菌所结根瘤。另外一些理化特性鉴定正在进行。
Fast-growing soybean rhizobia isolated from Liaoning showed two types on citrate media. Seven out of 12 strains did not grow in citrate medium and 5 grew well. But it grows slower than A. tumefaciens. None of the strains produced 3-keto lactose. In addition to a litmus milk on the other, have produced a serum ring, three acid, eight strains of a base is not obvious. 0.3 M-0.4 M NaCl is generally tolerant to salt, while 4 strains capable of utilizing citrate are resistant to 0.45 M Nacl. These strains and China’s cultivated soybean varieties Tiefeng 18, No. 8, No. 77-153 and other varieties can carry out effective symbiosis. However, the dry weight and nitrogen content of plants were significantly lower than those of slow-growing soybean rhizobia. Fast strains and Tiefeng 18, Kaiyu 8 good affinity, 77-153 poor. 111,121,123 A were connected to the cowpea (Beijing red) and mung bean (D0245-1), respectively, in addition to the rest of the two strains are nodulated and nitrogenase activity. All nodulation tests have been identified with the corresponding antiserum, to prove indeed by the fast-growing soybean rhizobia nodules. Other physical and chemical characterization is underway.