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目的探讨胰多肽在大鼠胃溃疡自愈期间的可能作用及意义。方法应用免疫组织化学PAP法、形态计量和图像分析法,研究大鼠胃溃疡自愈期间胰岛PP细胞的形态学、细胞的面数密度(NA)及平均灰度变化。结果与正常组和盐水组比较,胃溃疡组大鼠胰岛PP细胞NA于溃疡第6d明显下降,平均灰度值升高(P<0.05);溃疡第10-28d,PP细胞NA升高、平均灰度值下降(P<0.05);尤以第10d最明显。胰岛PP细胞免疫组织化学的形态观察结果与同期图像分析结果基本一致。结论胰岛PP细胞在实验性胃溃疡自愈期间,其细胞NA和平均灰度均发生改变。提示PP细胞参与了胃溃疡自愈的调节过程。
Objective To investigate the possible role and significance of pancreatic polypeptide in the healing of gastric ulcer in rats. Methods Immunohistochemical PAP method, morphometry and image analysis were used to study the morphological changes, number density (NA) and average gray level of pancreatic islet cells during healing of gastric ulcer in rats. Results Compared with normal group and saline group, NA of pancreatic islet cells in gastric ulcer group decreased significantly on the 6th day and the average gray value increased (P <0.05) Gray value decreased (P <0.05), especially in the first 10d most obvious. Immunohistochemistry of pancreatic islets PP morphologically observed with the same period of image analysis results are basically the same. Conclusion The pancreatic islets PP cells in the healing of experimental gastric ulcer, the cell NA and the average grayscale were changed. Tip PP cells involved in the regulation of gastric ulcer healing process.