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目的:通过对血管内皮生长因子-C(VEGF-C)和环氧化酶-2(COX-2)在青海地区藏族和汉族乳腺癌患者组织中的表达情况分别予以研究,分析两者在藏、汉族乳腺癌组织中表达的相关性及与临床病理特征的关系,并对藏、汉族乳腺癌组织中表达的VEGF-C和COX-2进行比较。方法:随机选取青海大学附属医院肿瘤外科接受根治性手术且具有详细病历的藏族、汉族乳腺癌组织蜡块各80例,制成组织芯片,采用免疫组化S-P法分别检测藏族和汉族乳腺癌组织当中VEGF-C和COX-2表达情况,对藏、汉族乳腺癌组织中VEGF-C和COX-2与乳腺癌临床病理特征指标的相关性进行相关统计学分析,并对两民族间乳腺癌患者组织中VEGF-C和COX-2的表达进行比较。结果:在随机选取的藏族和汉族乳腺癌组织标本中,VEGF-C、COX-2均呈高表达表现。80例汉族乳腺癌患者中,VEGF-C、COX-2阳性表达率分别为51.3%、52.5%,二者表达呈正相关(r=0.374,P<0.05),VEGF-C在汉族乳腺癌组织中的表达与临床分期、淋巴转移正相关,同时,COX-2蛋白的表达也与临床分期以及淋巴结转移相关;80例藏族乳腺癌患者中,VEGF-C、COX-2阳性表达率分别为70.0%、65.0%,二者表达呈正相关(r=0.206,P<0.05),VEGF-C在藏族乳腺癌组织中的表达与临床分期、淋巴转移呈正相关,COX-2蛋白的表达同样也与临床分期以及淋巴结转移相关。藏族乳腺癌患者组织中COX-2和VEGF-C表达明显高于汉族乳腺癌患者(P<0.05),具有显著统计学差异。结论:VEGF-C和COX-2在藏、汉族乳腺癌组织存在高表达且具有正相关关系,由此得出无论是汉族还是藏族乳腺癌患者,检测分析组织中VEGF-C和COX-2的表达情况对指导乳腺癌治疗和评估预后均具有重要意义。藏族乳腺癌患者组织中VEGF-C、COX-2表达值明显高于汉族乳腺癌患者表达值,因此,对藏族乳腺癌患者进行VEGF-C和COX-2检测可做到早期诊断和治疗的目的,这可能成为乳腺癌诊疗方面的热点。
Objective: To investigate the expression of VEGF-C and COX-2 in Tibetan and Han nationality breast cancer patients in Qinghai Province, , Han Chinese breast cancer tissues and its relationship with clinicopathological features, and to compare the expression of VEGF-C and COX-2 in Tibetan and Han nationality breast cancer tissues. Methods: Totally 80 cases of Tibetan and Han nationality breast cancer tissue wax blocks were randomly selected from Department of Oncology, Affiliated Hospital of Qinghai University for radical surgery and with detailed medical records. Tissue microarrays were made and immunohistochemical SP method was used to detect the expression of breast cancer in Tibetan and Han nationality VEGF-C and COX-2 expression in breast cancer in Tibetan and Han patients, the correlation between VEGF-C and COX-2 and clinicopathological features of breast cancer were statistically analyzed, and two ethnic patients with breast cancer The expression of VEGF-C and COX-2 in tissues was compared. Results: The expression of VEGF-C and COX-2 in Tibetan and Han nationality samples were highly expressed. The positive rates of VEGF-C and COX-2 in 80 Han patients were 51.3% and 52.5%, respectively (r = 0.374, P <0.05). The positive rate of VEGF-C in Han breast cancer The expression of COX-2 was also correlated with clinical stage and lymph node metastasis. The positive rates of VEGF-C and COX-2 in 80 Tibetan women with breast cancer were 70.0% , 65.0% respectively. There was a positive correlation between the expression of VEGF-C and clinical stage and lymph node metastasis in Tibetan women (r = 0.206, P <0.05). The expression of COX-2 protein was also correlated with clinical stage As well as lymph node metastasis. The expression of COX-2 and VEGF-C in Tibetan breast cancer patients was significantly higher than that in Han patients with breast cancer (P <0.05), with significant statistical difference. Conclusions: There is a positive correlation between the expression of VEGF-C and COX-2 in Tibetan and Han nationality breast cancer tissues. It is concluded that both VEGF-C and COX-2 Expression of the guidance of breast cancer treatment and prognosis are of great significance. The expression of VEGF-C and COX-2 in Tibetan patients with breast cancer is significantly higher than that in Han patients, therefore, the detection of VEGF-C and COX-2 in Tibetan patients with breast cancer can be used for early diagnosis and treatment , Which may become a hot spot in the diagnosis and treatment of breast cancer.