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目的:研究诺如病毒爆发与HBGAs受体的关系。方法:选择2010年1月-2011年12月两年期间诺如病毒爆发的患者60例,作为实验组,同时,随机选择同期健康志愿者60例,作为对照组。收集患者和志愿者唾液,采用凝集抑制实验法检测唾液中HBGAS血型物质;用EIA法检测NoV-VLP与HBGAs的结合特性;比较不同型别诺如病毒爆发在实验组和对照组中HBGAs分布差异。结果:在感染诺如病毒的患者中,并无患者是唾液非分泌型,提示非分泌型HBGAs受体不与GⅡ24型诺如病毒毒株结合与OD450值测定结果一致。其中检出分泌型中A型1例(1.67%),B型48例(80.00%),O型3例(5.00%),AB型2例(13.33%),60例患者的检测结果与其本身的血型相一致。无论是分泌型还是非分泌型的分布比例,两组存在明显的差异(P<0.05),具有统计学意义。结论:B型患者为诺如病毒感染的高度敏感人群,提示在我国人群的感染类型多以分泌型B型人群为主。
Objective: To study the relationship between Norovirus outbreaks and HBGAs receptors. Methods: Sixty patients with norovirus outbreaks from January 2010 to December 2011 were selected as the experimental group. Meanwhile, 60 healthy volunteers were randomly selected as the control group. The saliva of patients and volunteers were collected, agglutination inhibition test was used to detect HBGAS blood group substance in saliva. The binding characteristics of NoV-VLP and HBGAs were detected by EIA. Differences in the distribution of HBGAs in different types of Norovirus were compared between the experimental group and the control group . RESULTS: Of the patients infected with norovirus, none of the patients were non-salivary, suggesting that the non-secreted HBGAs receptor did not bind to norovirus GII24 and was consistent with the OD450 value. There were 1 case (1.67%) in type A, 48 (80.00%) in type B, 3 (5.00%) in type O and 2 (13.33%) in type AB in 60 cases Consistent with the blood type. There was a significant difference between the two groups (P <0.05), regardless of the proportion of secreted or non-secreted, with statistical significance. Conclusion: Type B patients are highly sensitive to Norovirus infection, suggesting that the majority of infection types in our population are mainly secretory type B patients.