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５－ＨＴ灌流幼年组及成年组大鼠脑片。幼年组６６．７％额叶后部Ⅳ层神经元的兴奋性突触后电位（ＥＰＳＰ）幅度降低７３．９％，膜电位和膜电阻无变化；此作用为５－ＨＴ１β激动剂ＴＦＭＰＰ模拟，为５－ＨＴ１Ａ／１β拮抗剂ｐｉｎｄｏｌｏｌ阻抑；５－ＨＴ不降低低钙高镁液中谷氨酸钠的去极化反应。提示幼年期５－ＨＴ经突触前膜５－ＨＴ１Ｂ受体抑制突触传递。５－ＨＴ灌流使成年组６０．９％神经ＥＰＳＰ降低３６．２％，伴随膜电阻减小；此作用为５－ＨＴ１Ａ激动剂８－ＯＨ－ＤＰＡＴ模拟、５－ＨＴ１Ａ拮抗剂ＮＡＮ－１９０阻抑；在低钙高镁液中５－ＨＴ轻度抑制谷氨酸钠的去极化反应，提示成年期５－ＨＴ主要经突触后膜５－ＨＴ１Ａ受体发挥抑制效应。上述发现表明５－ＨＴ对丘脑—皮质投射的抑制途径与年龄有关，幼年期５－ＨＴ１Ｂ受体发挥了阶段性的重要作用，成年期５－ＨＴ１Ａ受体是发挥作用的主要径路。
5-HT perfusion of juvenile and adult rat brain slices. The amplitude of excitatory postsynaptic potential (EPSP) decreased by 73.9% in neurons in 66.7% of the frontal lamina propria in young group, with no change in membrane potential and membrane resistance; this effect was a TFMPP simulation of 5-HT1β agonist, 5-HT1A / 1β antagonist pindolol suppression; 5-HT does not reduce the low-calcium high magnesium solution of sodium glutamate depolarization. Tip juvenile 5-HT presynaptic membrane 5-HT1B receptor inhibition of synaptic transmission. 5-HT perfusion reduced 60.9% of neural EPSP in adult group by 36.2%, with the decrease of membrane resistance; this effect was simulated by 5-HT1A agonist 8-OH-DPAT and inhibited by 5-HT1A antagonist NAN-190 ; 5-HT in low calcium high magnesium solution mildly inhibited the depolarization of sodium glutamate, suggesting that adult 5-HT mainly by the postsynaptic membrane 5-HT1A receptor play a inhibitory effect. These findings indicate that 5-HT inhibition of thalamic-cortical projection and age-related, juvenile 5-HT1B receptors play a phased important role in adult 5-HT1A receptor is the main path to play a role.