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采用离体生测的方法测定了F500、氟吗·锰锌和甲霜灵对大豆疫霉菌野生型敏感菌株及抗甲霜灵菌株的抑制作用。结果表明,3种药剂对敏感菌株的菌丝生长、孢子囊的形成、游动孢子的形成和释放及卵孢子的形成都有较明显的抑制作用,F500和氟吗·锰锌的抑制作用优于甲霜灵,F500和氟吗·锰锌对抗性菌株的菌丝生长抑制作用明显,甲霜灵对抗性菌株的菌丝生长抑制作用不明显;药剂浓度为50μg·mL-1时,F500和氟吗·锰锌对抗性菌株的抑制率分别为97.9%和100%,EC50为0.0002μg·mL-1和0.5525μg·mL-1,甲霜灵对抗性菌株的抑制率为仅为60%,EC50为8.5024μg·mL-1,甲霜灵与F500和氟吗·锰锌无交互抗性。
In vitro bioassay method was used to determine the inhibitory effect of F500, Fluoro-manganese and zinc and metalaxyl on the wild type sensitive strains and metalaxyl-resistant strains of Phytophthora sojae. The results showed that the three agents had obvious inhibitory effect on mycelial growth, sporocyst formation, zoospore formation and release and oospores formation of the susceptible strains. The inhibitory effect of F500 and Fluoro-Mn-Zn was superior In the metalaxyl, F500 and flumorph · Mn-Zn antagonistic strains mycelial growth inhibition obvious, metalaxyl antagonistic mycelial growth inhibition was not obvious; drug concentration of 50μg · mL-1, F500 and The inhibitory rates of fluoro-manganese-zinc antagonistic strains were 97.9% and 100%, respectively. The EC50 was 0.0002μg · mL-1 and 0.5525μg · mL-1, the inhibition rates of metalaxyl-resistant strains were only 60% EC50 of 8.5024μg · mL-1, metalaxyl and F500 and flumorph · Mn-Zn no cross-resistance.