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目的 探讨p38的4种亚型在细胞中的分布以及对外界刺激的反应。方法 应用激光共聚焦显微镜对RAW264.7单核细胞内的4种亚型进行定位观察。结果 p38(、p38(在未受刺激的静息细胞内的荧光强度呈弥散性分布，受脂多糖（LPS）刺激后，细胞核荧光强度明显增强，细胞浆荧光强度降低，提示LPS诱导p38(和p38(磷酸化活化后移位入细胞核。p38(在静息和受LPS刺激后的细胞中均呈散在、弥漫性地分布，提示p38(刺激后无移位现象。静息时，p38(弥散地分布于细胞，刺激后细胞核膜部荧光强度增高，提示受刺激后p38(移位于核膜周围。结论 p38不同亚型在细胞受LPS刺激后移位表现不同，可能与它们在组织和细胞分布的特异性及作用途径有关。
Objective To investigate the distribution of four subtypes of p38 in cells and the response to external stimuli. Methods Four subtypes of RAW264.7 mononuclear cells were observed by laser confocal microscopy. Results The fluorescence intensity of p38 (, p38 (p38) was diffusely distributed in unstimulated resting cells. After stimulated by lipopolysaccharide (LPS), the fluorescence intensity of nucleus was significantly increased and the fluorescence intensity of cytoplasm decreased. p38 (phosphorylation of activated translocation into the nucleus. p38 (scattered in the resting and LPS-stimulated cells are scattered, diffuse distribution, suggesting that p38 (no shift after stimulation at rest, p38 (diffuse And the fluorescence intensity of the nuclear membrane increased after stimulation, suggesting that p38 (shifted to the periphery of the nuclear membrane) after stimulation.Conclusion The different subtypes of p38 may show different displacements when stimulated by LPS, which may be related to their expression in tissues and cells Distribution of specificity and role of pathways.