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目的:观察蝎毒素(ITX)对Lewis肺癌生长及对荷瘤小鼠免疫细胞的影响。方法:建立Lewis肺癌荷瘤小鼠模型后,随机分为模型组、rIL-2组、ITX组、空白组,每组8只小鼠,接种第2日起,每天腹腔注射给药,连用16天。末次给药24h后,用流式细胞术检测小鼠外周血中免疫细胞的功能状态;酶联免疫法检测小鼠外周血TGF-β1水平,并测量瘤体质量和体积。结果:ITX干预后荷瘤小鼠单核、NK和B细胞均有一定活化,但无统计学意义;rIL-2干预后荷瘤小鼠的NK和B细胞有明显活化(P<0.05);ITX组和rIL-2组血清TGF-β1水平均高于模型组,rIL-2组明显高于模型组(P<0.05);ITX和rIL-2干预后瘤体质量和体积均有增加,ITX组明显高于模型组(P<0.05)。结论:ITX和rIL-2均促进了瘤体质量和体积的增加,可能与其活化的免疫细胞有关。
Objective: To observe the effects of scorpion toxin (ITX) on the growth of Lewis lung carcinoma and the immune cells of tumor-bearing mice. Methods: Lewis lung cancer tumor-bearing mice model was established and randomly divided into model group, rIL-2 group, ITX group and blank group. Each group of 8 mice were given intraperitoneal injection once a day from the second day of inoculation, day. 24h after the last administration, the functional status of immune cells in peripheral blood of mice was detected by flow cytometry; the level of TGF-β1 in peripheral blood of mice was measured by enzyme-linked immunosorbent assay and the mass and volume of tumor were measured. Results: The single nucleus, NK and B cells in tumor-bearing mice had some activation after ITX intervention, but there was no statistical significance. NK and B cells in tumor-bearing mice were significantly activated after rIL-2 treatment (P <0.05). The serum levels of TGF-β1 in ITX group and rIL-2 group were significantly higher than those in model group, while those in rIL-2 group were significantly higher than those in model group (P <0.05). The ITX and rIL-2 groups both increased the mass and volume of ITX Group was significantly higher than the model group (P <0.05). Conclusion: Both ITX and rIL-2 promote the increase of tumor mass and volume, which may be related to the activated immune cells.