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以具典型晚疫病症状的番茄病叶为试材,采用薯片夹叶分离法对病原菌进行分离,测定了不同培养基、pH、温度、碳源、氮源对菌丝生长、产孢的影响,通过病原菌鉴定和生物学特性研究,以期为西藏设施番茄晚疫病发生及防治提供理论依据。结果表明:引起设施番茄晚疫病的病原菌为致病疫霉菌Phytophthora infestans(Mont)de Bary。病菌菌丝在大多数培养基上均能良好生长,其中RSA培养基最适宜菌丝生长及产孢;菌丝生长和产孢的最适温度范围为18℃,最适生长pH 6~7;菌丝对碳源的利用,以可溶性淀粉最好,麦芽糖最差;在氮源利用方面,菌丝在以脯氨酸为氮源的查氏培养基上生长最快,在精氨酸、胱氨酸和氯化铵上不生长。孢子囊的致死温度为55℃,15min。
The pathogen was isolated from potato leaves with typical late blight symptoms. The pathogen was isolated from the leaves of potato crisps, and the effects of different media, pH, temperature, carbon source and nitrogen source on mycelial growth and sporulation were measured , Through the identification of pathogenic bacteria and biological characteristics, with a view to provide a theoretical basis for the occurrence and prevention of tomato late blight in Tibet facilities. The results showed that the pathogen causing Phytophthora infestans (Mont) de Bary was Phytophthora infestans. The mycelial growth of mycelium was most suitable for mycelial growth and sporulation. The optimum temperature for mycelium growth and sporulation was 18 ℃, and the optimum pH was 6-7. Mycelium on carbon utilization, the best soluble starch, maltose worst; in the use of nitrogen, the mycelium in Proline as the nitrogen source of Charles growth medium fastest growth in arginine, cyst No growth on amino acids and ammonium chloride. The lethal temperature of sporangia is 55 ℃, 15min.