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目的 探讨胃粘膜活检标本端粒酶和增殖细胞核抗原 (PCNA)的表达意义。方法 采用TRAP法及免疫组织化学方法对 12 2例胃粘膜活检标本端粒酶活性及PCNA表达情况进行同步检测。结果 胃癌组端粒酶阳性率为 88 1% (6 0 / 6 8) ,明显高于慢性浅表性胃炎组 (0 / 2 6 )及慢性萎缩性胃炎组 (4 / 18)。端粒酶表达阳性者胃癌组细胞增殖活性为 (82 9± 16 8) % ,明显高于端粒酶表达阴性组的 (6 3 8± 15 1) % (P <0 0 1)。端粒酶阳性率与PCNA计数值在胃癌组呈良好的协同性。结论 在胃粘膜活检标本中联合检测端粒酶及PCNA对阐明胃癌的发生发展 ,监测癌前病变及早期诊断胃癌有非常重要的意义
Objective To investigate the expression of telomerase and proliferating cell nuclear antigen (PCNA) in gastric biopsy specimens. Methods TRAP and immunohistochemistry were used to detect telomerase activity and PCNA expression in 12 gastric biopsy specimens simultaneously. Results The positive rate of telomerase in gastric cancer group was 88.1% (60/68), which was significantly higher than that in chronic superficial gastritis group (0/26) and chronic atrophic gastritis group (4/18). The positive rate of telomerase expression in gastric cancer group was (82 9 ± 16 8)%, which was significantly higher than that in the negative group (63 8 ± 15 1)% (P 0 01). The positive rate of telomerase and PCNA count showed good synergy in gastric cancer group. Conclusion Combined detection of telomerase and PCNA in gastric mucosal biopsy specimens is of great significance in elucidating the occurrence and development of gastric cancer, monitoring precancerous lesions and early diagnosis of gastric cancer