通过对家蝇神经系统ＮａＫＡＴＰａｓｅ性质的研究，表明ＮａＫＡＴＰａｓｅ反应的适宜ｐＨ值为７０～８０，适温为３５～４０℃，Ｋｍ为０２２ｍｍｏｌ／Ｌ，Ｖｍａｘ为５５５５６ｎｍｏｌ／（ｍｇ·ｍｉｎ）。比较测定了家蝇敏感品系、ＤｅｌＲ、２ＣｌＲ抗性品系的ＮａＫＡＴＰａｓｅ活性及溴氰菊酯和氯菊酯对该酶的抑制作用。实验证明，敏感与抗性品系间ＮａＫＡＴＰａｓｅ活力无显著的差异，但溴氰菊酯和氯菊酯对不同家蝇品系ＮａＫＡＴＰａｓｅ的抑制作用有明显区别，两种拟除虫菊酯可抑制敏感家蝇品系ＮａＫＡＴＰａｓｅ的活性，而对抗性品系无明显的抑制作用。 Through the research on the properties of NaKATPase in the nervous system of housefly, the suitable pH value of NaKATPase reaction was 7.080, suitable temperature was 35-40 ℃, Km was 0.22mmol / L , Vmax 555 56nmol / (mg · min). The activity of NaKATPase in DelR and 2ClR resistant strains of housefly susceptible strains and the inhibitory effect of deltamethrin and permethrin on this enzyme were compared. Experiments show that there is no significant difference in NaKATPase activity between sensitive and resistant strains, but deltamethrin and permethrin have significant differences on the inhibitory effect of NaKATPase on different strains of domestic flies. Two kinds of pyrethrum Ester inhibited the activity of NaKATPase in sensitive housefly lines, while the antagonistic lines had no obvious inhibitory effect.