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目的:研究红景天苷对力达霉素导致DNA损伤后所诱导的细胞衰老的调节作用。方法:采用MTT法测定药物对人胚肺二倍体成纤维细胞2BS活力的影响;衰老相关的β-半乳糖苷酶染色法观察细胞衰老形态变化;流式细胞术检测细胞周期分布以及γ-H2AX蛋白表达变化;蛋白免疫印迹技术观察p53和p21蛋白表达水平变化。结果:较低浓度(低于0.1 nmol.L-1)力达霉素能够抑制2BS细胞增殖,诱导β-半乳糖苷酶染色率显著升高和G2/M期阻滞,并使DNA损伤相关的γ-H2AX蛋白以及p53和p21蛋白表达升高。红景天苷能够显著干预力达霉素诱导的上述细胞衰老样表型及相关分子信号变化。结论:红景天苷能够抑制基因毒药物所致DNA损伤及其诱导的细胞衰老。
AIM: To investigate the regulatory effect of salidroside on cellular senescence induced by DNA damage induced by lidamycin. Methods: MTT assay was used to determine the effect of drugs on the 2BS activity of human embryo lung diploid fibroblasts. Aging-related β-galactosidase staining was used to observe the morphological changes of cells. Flow cytometry was used to detect cell cycle distribution and γ- H2AX protein expression changes; Western blot analysis of p53 and p21 protein expression levels. Results: Lidamycin at a lower concentration (less than 0.1 nmol.L-1) could inhibit the proliferation of 2BS cells, induce a significant increase of β-galactosidase staining rate and G2 / M phase arrest, and lead to DNA damage Γ-H2AX protein and p53 and p21 protein expression increased. Salidroside can significantly interfere with leptomycin-induced cellular senescence-like phenotypes and related molecular changes. Conclusion: Salidroside can inhibit DNA damage induced by genotoxic drugs and its induced cell senescence.