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目的 :了解河南省肠出血性大肠杆菌 (enterohemorrhagicescherichiacoli,EHEC)的流行状况与流行菌株的志贺毒素基因型。方法 :采集河南省 14个监测点上腹泻病人、家畜家禽粪便和肉食品等标本分离E HEC菌株。应用分子生物学技术检测EHEC菌株的rfbO157、rfbO111、hlyA、eaeA、stx2 和stx1等毒力基因和志贺毒素基因亚型 ,应用细胞培养技术检测菌株Vero细胞毒性。结果 :从腹泻病人、家畜家禽、肉食品、苍蝇、蜣螂中均分离出EHECO15 7:H7菌株 ,波尔山羊的带菌率最高达到 2 9.8%。 13 8株携带志贺毒素基因的菌株中 ,EHECO15 7:H7计 95株 ,占产毒菌株的 68.8% ;EHEC非O15 7菌株 43株 ,占 3 1.2 %。菌株可分为 5种毒素基因型 ,EHECO15 7:H 7仅含stx2 基因 ,以stx2 vha亚型为主 ,不含有stx1基因。EHEC非O15 7血清型毒素基因型较复杂。携带stx1和 /或stx2 的菌株均具有Vero细胞毒性。结论 :河南省腹泻病人和家畜家禽中存在EHEC感染。EHECO15 7:H7血清型是优势菌型 ,羊是主要宿主动物。目前河南省EHECO15 7:H7菌株毒素基因型为stx2 并以stx2 vha亚型为主。
Objective: To understand the prevalence of enterohemorrhagicescherichia coli (EHEC) in Henan Province and the genotype of Shiga toxin in the epidemic strains. Methods: EHEC strains were collected from diarrhea patients, livestock and poultry manure and meat products in 14 monitoring sites in Henan Province. The virulence genes of rfbO157, rfbO111, hlyA, eaeA, stx2 and stx1 and the shiga toxin gene subtypes of EHEC strains were detected by molecular biology techniques. The cytotoxicity of strain Vero was detected by cell culture technique. Results: The EHECO15 7: H7 strain was isolated from diarrhea patients, domestic fowls, meat products, flies and dung beetles. The highest rate of carrying bacteria in Boer goats reached 29.8%. Of the 13 strains carrying Shiga toxin genes, 95 strains of EHECO15 7: H7 accounted for 68.8% of the producing strains; 43 strains of EHEC non-O15 7 strains accounted for 32.2%. Strain can be divided into five toxin genotypes, EHECO15 7: H7 only contains stx2 gene, mainly stx2 vha subtype, does not contain stx1 gene. EHEC non-O15 7 serotype toxin genotypes more complicated. Strains carrying stx1 and / or stx2 all have Vero cytotoxicity. Conclusion: There are EHEC infections in diarrhea patients and livestock in Henan Province. EHECO15 7: H7 serotype is dominant bacteria, sheep is the main host animal. At present, Henan province EHECO15 7: H7 strain toxin genotype stx2 stx2 vha subtype based.