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本文报道用干燥的带血斑纸片,测定庆大霉素类的简便方法。庆大霉素广泛用于治疗革兰氏阴性细菌的感染,要获得最佳的疗效、避免毒副作用和防止治疗的失败,监测药物的血清水平是很重要的。作者提出的方法是:滴加100微升含庆大霉素的血液于滤纸片上,在50℃气流下干燥或在室温下凉干。置血斑纸片于超滤管里,加500微升0.5mol/LNa_2HPO_4溶液,在35℃保温60分钟,将滤纸的洗脱液离心,移入Abott TDX药筒,再进行荧光极化免疫测定。用这个方法能最有效地从血斑里回收庆大霉素。在滤纸血斑里,庆大霉素在室温下可稳
This article reports a simple method for the determination of gentamycin using dried blotted paper. Gentamicin is widely used in the treatment of Gram-negative bacterial infections. It is important to monitor the serum level of the drug for optimal efficacy, avoidance of side effects, and prevention of treatment failure. The authors suggest that: 100 microliters of gentamicin-containing blood is added dropwise to the filter paper, dried at 50 ° C under air or dry at room temperature. Place blood spot paper in the ultrafiltration tube, add 500 microliters of 0.5 mol / L Na 2 HPO 4 solution and incubate at 35 ℃ for 60 minutes. Centrifuge the filter paper eluent and transfer to Abott TDX cartridge for fluorescence polarization immunoassay. With this method, gentamicin can be recovered most effectively from blood spots. In filter paper Xue Ge, gentamicin can be stable at room temperature