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采用超声破碎，ＴｒｉｔｏｎＸ─１００处理和Ｓｅｐｈａｃｒａｌ超细Ｓ─３００凝胶过滤技术提取了鼠伤寒杆菌的主要外膜蛋白（ＭＯＭＰｓ）。ＭＯＭＰｓ的脂多糖（ＬＰＳ）含量约为０．２％。经ＳＤＳ─ＰＡＧＥ图谱显示蛋白在３６─４１ＫＤ之间。ＭＯＭＰｓ能使小鼠产生典型的足垫肿胀（ＤＴＨ）及高水平的ＩＬ─２；可保护５００ＬＤ５０鼠伤寒杆菌及伤寒杆菌的攻击，其免疫保护率分别为９０％和３３．３％，用５０ｕｇＭＯＭＰｓ免疫的小鼠的Ｔ淋巴细胞经尾静脉注射给非免疫小鼠，可使后者得到被动免疫保护，其保护率为４２．９％。基于上述实验结果，本文认为鼠伤寒杆菌的ＭＯＭＰｓ是一良好的保护性抗原。
The major outer membrane proteins (MOMPs) of Salmonella typhimurium were extracted by ultrasonic disruption, Triton X-100 treatment and Sephacral ultra-fine S-300 gel filtration technology. MOMPs have lipopolysaccharide (LPS) levels of about 0.2%. The SDS-PAGE showed that the protein was between 36-41KD. MOMPs can cause typical footpad swelling (DTH) and high levels of IL-2 in mice, which can protect against 500LD50 Salmonella typhimurium and Salmonella typhi, with immunoprotection rates of 90% and 33.3%, respectively. Immunization of T lymphocytes in mice through the tail vein injection of non-immune mice, the latter can be passive immune protection, the protection rate was 42.9%. Based on the above experimental results, we conclude that MOMPs of Salmonella typhimurium is a good protective antigen.