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[目的]探讨砷染毒对大鼠雌激素及其受体的影响和雌激素受体(ER)信号通路指标间的关系。[方法]以雌性SD大鼠为实验动物,随机分成4组,每组6只。用亚砷酸钠灌胃染毒。分别为:对照组、低砷组(0.625 mg/kg)、中砷组(2.500 mg/kg)、高砷组(10.000 mg/kg)。每天1次灌胃染毒,连续3个月。染毒结束用酶联免疫吸附法测定大鼠血清中卵泡刺激素(FSH)、促黄体生成素(LH)、雌二醇(E2)及ER的水平。[结果]对照组、低砷组、中砷组、高砷组FSH表达水平分别为:(26.05±2.10)、(18.23±1.58)、(10.22±1.58)、(2.07±0.79)IU/L;LH表达水平分别为:(39.43±1.65)、(10.33±2.08)、(1.95±0.29)、(0.60±0.23)IU/L;E2表达水平分别为:(54.92±5.14)、(40.68±3.01)、(9.20±1.92)、(9.03±1.02)ng/L;ER表达水平分别为:(41.06±2.14)、(24.32±2.21)、(22.16±3.16)、(9.06±2.06)ng/L。随着砷染毒剂量的增高,FSH、LH表达逐渐降低(P<0.01);除E2在砷中、高染毒剂量(2.500、10.000 mg/kg),ER在砷低、中染毒剂量(0.625、2.500 mg/kg)的表达水平差异无统计学意义(P>0.05)外,其余剂量间表达差异均有统计学意义(P<0.01)。尿砷与FSH、LH、E2、ER间存在剂量-效应关系,砷、尿砷与FSH、LH、E2、ER均为负相关关系(P<0.05);ER信号通路指标间FSH、LH与E2,E2与ER间均为正相关关系(P<0.05)。[结论]雌激素及其受体参与了砷暴露致大鼠生殖系统的损伤,砷抑制ER通路各指标的表达,砷很可能是内分泌干扰物。
[Objective] To investigate the effect of arsenic exposure on estrogen and its receptor and estrogen receptor (ER) signaling pathway in rats. [Method] Female SD rats were used as experimental animals and randomly divided into 4 groups with 6 in each. Irritation with sodium arsenite exposure. They were: control group, low arsenic group (0.625 mg / kg), middle arsenic group (2.500 mg / kg) and high arsenic group (10.000 mg / kg). Gavage once a day for 3 months. Serum levels of follicle stimulating hormone (FSH), luteinizing hormone (LH), estradiol (E2) and ER were measured by enzyme-linked immunosorbent assay (ELISA) [Results] The expression of FSH in control group, low arsenic group, middle arsenic group and high arsenic group were (26.05 ± 2.10), (18.23 ± 1.58), (10.22 ± 1.58), (2.07 ± 0.79) IU / The expression levels of LH were (39.43 ± 1.65), (10.33 ± 2.08), (1.95 ± 0.29) and (0.60 ± 0.23) IU / L, respectively. The expressions of E2 were 54.92 ± 5.14 and 40.68 ± 3.01, , (9.20 ± 1.92) and (9.03 ± 1.02) ng / L respectively. The expression levels of ER were (41.06 ± 2.14), (24.32 ± 2.21), (22.16 ± 3.16) and (9.06 ± 2.06) ng / L, respectively. With the increase of arsenic dose, the expression of FSH and LH gradually decreased (P <0.01). Except for E2 in arsenite, high exposure dose (2.500,10.000 mg / kg) 0.625, 2.500 mg / kg), there was no significant difference (P> 0.05) between the other doses. There was a dose-effect relationship between urinary arsenic and FSH, LH, E2, ER, arsenic, urinary arsenic and FSH, LH, E2 and ER were negatively correlated (P <0.05); ER signaling pathway between FSH, LH and E2 , E2 and ER were positive correlation (P <0.05). [Conclusion] Estrogen and its receptor are involved in the damage of reproductive system in rats exposed to arsenic, and arsenic inhibits the expression of various ER signaling pathways. Arsenic may be an endocrine disruptor.