【摘 要】
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SMALL RNA IN REVERSE TRANSCRIPTASE TOO SMALL TO BE FOUNDSignificant background in no-template or no-primer RT-PCR is frequently noted.However,the underlying mec
【机 构】
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Jiangsu Engineering Research Center for microRNA Biology and Biotechnology, State Key Laboratory of
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SMALL RNA IN REVERSE TRANSCRIPTASE TOO SMALL TO BE FOUNDSignificant background in no-template or no-primer RT-PCR is frequently noted.However,the underlying mechanism is still unclear.In this issue,Jie Xu and colleagues reveal that the commercial reverse transcriptases contain small RNAs.In AMV reverse transcdptase,they detected microRNAs dedved from the virus host chick or other birds.This surprising finding at least partially explains the high background in some AMV reverse transcdptases based RT-PCR,especially for quantifying those microRNAs.Interestingly,they also found that the recombinant M-MLVs contain small RNAs derived from E.coil These findings suggest that small RNAs may be co-purified with the enzymes during their producdon,which is likely to interfere with RNA quantitation by using it as a template or RNA primer for reverse transcription or other unknown mechanisms.The co-purified RNA might come from the non-specific binding of small RNA without appropriate treatment with RNase or the RNA binding activity of reverse transcdptase.
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