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DNA条形码技术是利用基因组中一段短的标准序列进行物种鉴定及探索其亲缘进化关系的方法。本研究对采自海南不同地区降香黄檀5个居群24份样品的psbA-trnH、rbcL、核ITS及ITS2序列进行PCR扩增和测序,比较各序列扩增和测序效率。采用BLAST1和邻接(NJ)法构建系统聚类树方法评价不同序列的鉴定能力。结果表明ITS2在所研究的材料中具有最高的扩增和测序效率,而ITS扩增效率较低。ITS2完整序列在区分黄檀属不同种间差异具有较大优势。因此可利用ITS2从分子水平区分降香黄檀与其他混伪种。
DNA barcoding is a method of identifying species and exploring their phylogenetic relationships using a short set of standard sequences in the genome. In this study, psbA-trnH, rbcL, nuclear ITS and ITS2 sequences of 24 samples collected from five populations of Dalbergia rosewood in different areas of Hainan Island were amplified by PCR and sequenced. The efficiency of amplification and sequencing of each sequence was compared. BLAST1 and adjacent (NJ) were used to construct the phylogenetic tree to evaluate the identification ability of different sequences. The results showed that ITS2 had the highest amplification and sequencing efficiency in the studied materials, while ITS amplification efficiency was lower. The ITS2 complete sequence has great advantages in distinguishing the different species of Dalbergia. Therefore, ITS2 can be used to distinguish Rhododendron from other pseudo-species from the molecular level.