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目的通过观察细胞程序性死亡ProgrammedcelldeathPCD在腭突发育及腭裂形成中的差异相关基因bcl-2的表达探讨两者在腭裂发生中的意义方法利用建立的腭裂模型用原位末端转移酶标记、原位杂交技术检测PCD的发生和bcl-2基因表达变化结果实验组小鼠在腭突垂直生长期、上抬期中PCD明显多于对照组p<0.01且bcl-2的表达相应增高两者之间具有密切的相关性p<0.01结论PCD处于一个复杂的网络式调控系统bcl-2基因的抗凋亡作用与其它相关基因共同协同实施对细胞的调控决定细胞的最终命运
OBJECTIVE: To investigate the expression of bcl-2 in the development of cleft palate and the expression of bcl-2 in the process of palatal degeneration and cleft palate development.Methods The cleft palate model was established by in situ terminal transferase Bit hybridization detection of PCD and bcl-2 gene expression changes in the experimental group mice in the vertical palatal growth period, the PCD was significantly higher than the control group p <0.01 and the corresponding increase in bcl-2 between the two With a close correlation p <0.01 Conclusion PCD in a complex networked regulatory system The anti-apoptotic effect of the bcl-2 gene and other related genes in conjunction with the implementation of the regulation of cells determines the ultimate fate of cells