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目的:研究海南汉族高血压及高血压脑梗塞与血管紧张素转换酶(ACE)基因多态性的关系。方法:采用聚合酶链反应(PCR)方法,对海南汉族146例正常人、106例高血压患者和227例高血压脑梗塞患者的ACE基因插入/缺失(I/D)多态性进行检测,观察DD、DI、II基因型频率及其等位D、I基因频率,并对所有普通PCR定为DD型的样本进行插入特异性PCR检测,以减少误分型率,并分析高血压患者及脑梗塞患者的经典危险因素。结果:海南汉族高血压组、高血压脑梗塞组与正常对照组进行比较,三组的DD,DI、II基因频率及D、I等位基因频率无显著性差异。结论:ACE基因I/D多态性与海南汉族正常人、高血压及高血压脑梗塞无显著关联,说明ACE插入/缺失多态性不是海南汉族高血压及高血压脑梗塞的独立危险因素。
Objective: To investigate the relationship between hypertension and hypertensive cerebral infarction and angiotensin-converting enzyme (ACE) gene polymorphism in Han Chinese. Methods: Polymerase chain reaction (PCR) was used to detect the ACE gene insertion / deletion (I / D) polymorphisms in 146 normal subjects, 106 hypertensive patients and 227 hypertensive cerebral infarction patients in Hainan Han nationality. The frequency of DD, DI, and II genotypes and their D, I gene frequencies were observed, and all the PCR-specific DD samples were inserted into specific PCR to reduce the misdiagnosis rate. Classical risk factors for patients with cerebral infarction. Results: There was no significant difference in frequencies of DD, DI, II and D, I alleles among Han nationality hypertension group, hypertensive cerebral infarction group and normal control group. Conclusion: ACE gene I / D polymorphism is not significantly associated with normal Han population, hypertension and hypertensive cerebral infarction in Hainan Han population, indicating that ACE insertion / deletion polymorphism is not an independent risk factor for hypertension and hypertensive cerebral infarction in Hainan Han population.