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目的:探讨痰咳净不同剂型(溶液剂、粉雾剂、散剂)体外抗甲型流感病毒作用,以及剂型与作用的关系。方法:以利巴韦林作阳性对照药物,采用存留细胞结晶紫染色法测定痰咳净提取液(溶液剂)不同给药方式对甲型人流感病毒H3N2的体外抑制作用,确定具体作用方式;并以有效的给药方式对比研究痰咳净不同剂型的抗病毒作用;再运用血清药效学方法比较痰咳净不同剂型的大鼠血清样本的体外抗病毒作用。结果:痰咳净溶液剂有一定的抗甲型人流感病毒的作用,包括综合抑制作用和抑制病毒吸附后的复制增殖作用,其最高抗病毒有效率为利巴韦林注射液的66.40%。溶液剂、粉雾剂和散剂在质量浓度为0.062g/mL时的抗流感病毒的有效率分别为43.50%、41.50%和37.36%。血清药效学研究结果表明药物溶出较快的剂型药物吸收较多,血清抗病毒药效较好。结论:痰咳净不同剂型均有一定的体外抗甲型人流感病毒作用,粉雾剂效果优于散剂。
OBJECTIVE: To investigate the anti-influenza A virus effect of different dosage forms of Phlegm-Cough (solution, powder aerosol and powder) in vitro and the relationship between dosage form and action. Methods: Ribavirin was used as a positive control drug, and the crystal violet staining method of surviving cells was used to determine the inhibitory effect of different phlegm cough net extract (solution) on human influenza A virus H3N2 in vitro and to determine the specific mode of action. To compare the antiviral effects of different formulations of Phlegm-Cough with effective administration methods; and then compare the antiviral effects of the different serum samples of Phlegm- Results: There was a certain anti-influenza A virus effect on sputum cough net solution, including the combined inhibition and inhibition of replication proliferation after virus adsorption, and the highest antiviral efficiency was 66.40% of ribavirin injection. The effective rates of anti-influenza virus solutions, powders and powders at the mass concentration of 0.062 g / mL were 43.50%, 41.50% and 37.36%, respectively. Serum pharmacodynamic studies have shown that drug-eluting drug formulations absorb more quickly, and serum antiviral efficacy is better. Conclusion: The different dosage forms of phlegm cough all have a certain anti-influenza A virus effect in vitro. The effect of powder aerosol is better than powder.