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目的:探讨热休克大鼠小脑小脑肽1(Cbln1)和孤独谷氨酸受体-2(Glud2)表达的变化及意义。方法:60只SD大鼠随机分为3组:空白组,休克早期组和休克晚期组。大鼠置于恒温恒湿箱模拟湿热天气。生理记录仪检测大鼠生命体征。ELISA检测小脑组织中Cbln1和血清中降钙素基因相关肽(CGRP)含量,流式细胞仪检测Glud2表达,放射免疫分析法检测血清中肾上腺素(E)、去甲肾上腺素(NE)、血管紧张素-Ⅱ(AngⅡ)、肾素(RA)浓度。结果:休克早期组和休克晚期组Cbln1和Glud2含量显著高于空白组(P<0.05);休克晚期组Cbln1和Glud2含量显著高于休克早期组(P<0.05);相关性分析显示Cbln1、Glud2分别与E、NE、AngⅡ、RA呈正相关(P<0.05);Cbln1和Glud2与CGRP无明显相关性(P>0.05)。结论:热休克大鼠Cbln1和Glud2表达增加,它们可能是自身抗休克机制之一。
Objective: To investigate the changes and significance of cerebellar cerebellar peptide 1 (Cbln1) and lonely glutamate receptor 2 (Glud2) in heat shock rats. Methods: Sixty SD rats were randomly divided into three groups: blank group, early shock group and shock late group. Rats were placed in a constant temperature and humidity box to simulate hot and humid weather. Physiological Logger for Detecting Rat Vital Signs. ELISA was used to detect Cbln1 in cerebellum and CGRP in serum, Glud2 expression was detected by flow cytometry, radioimmunoassay was used to detect serum levels of epinephrine (E), norepinephrine (NE), blood vessels Angiotensin II and renin (RA) concentrations. Results: The levels of Cbln1 and Glud2 in early shock stage and late shock stage were significantly higher than those in blank group (P <0.05), while the levels of Cbln1 and Glud2 in late shock stage were significantly higher than those in early shock stage (P <0.05). Correlation analysis showed that Cbln1, Glud2 (P <0.05). There was no significant correlation between Cbln1, Glud2 and CGRP (P> 0.05). Conclusion: Heat shock rats Cbln1 and Glud2 expression increased, they may be self-anti-shock mechanism.