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目的:探讨取样数量对药材含量检测结果的影响。方法:以柔毛淫羊藿和心叶淫羊藿为样本,确定单株,以及5,10,20,30,50株个体混合6个取样数量级别,采用HPLC的方法,测定朝藿定C和淫羊藿苷的含量以及朝藿定A、朝藿定B、鼠李糖基淫羊藿次苷Ⅱ和宝藿苷Ⅰ等4个成分的峰面积。结果:同一取样数量级别各成分的3个重复测定值的RSD值以及其中一次的测量值偏离假定真实值(50株个体混合的平均值)的最大偏差(最大变异率)随着取样个体数的增加而呈现出下降的趋势,但是不同药材、不同成分因取样数量不同产生的变化是有差异的。结论:进行淫羊藿药材含量测定时,研究样本的取样个体数不应少于10株,而取样个体数在50株以上时则更能代表研究样本的真实含量。
Objective: To investigate the effect of sampling quantity on the test results of medicinal materials. Methods: Samples of individuals and 5, 10, 20, 30, and 50 individuals were mixed and sampled at the level of epicarinous Epimedium and Epimedium epimedium as samples. The content of icariin and the peak areas of four components such as chlorhexidine A, chlorhexidine B, rhamnoyl icariin II, and arachidrin I. RESULTS: The RSD values of the three replicates of each component at the same sample size level and the maximum deviation (maximum variability) of the one-time measurement from the assumed true value (average of the 50 individual mixtures) along with the number of individuals sampled The increase shows a downward trend, but there are differences in the changes of different medicinal materials and different components due to different sampling quantities. Conclusion: The number of samples taken in the study sample should not be less than 10, and the number of sampled individuals above 50 should be more representative of the true content of the study sample.