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毛发是一种高度角化的材料,这类材料中含有二硫键,并含有硅、钙等元素,其主要特点是硬度较高,在超薄切片时,如使用常规的制样方法处理,会由于渗透不好,容易产生撕裂现象,很难得到满意的切片.我们在实际工作中摸索和改进了毛发的制备方法:1、使用疏基乙酸软化;2、OsO_4固定液浓度从原来的1%增加到2%;3、各步脱水、渗透时间延长;4、用平板定位包
Hair is a highly keratinized material, which contains disulfide bonds, and contains silicon, calcium and other elements, the main feature is the higher hardness, the ultra-thin slices, such as the use of conventional sample preparation methods, Will be due to poor penetration, prone to tearing, it is difficult to get satisfactory slices.We in the actual work to explore and improve the method of preparation of hair: 1, the use of sparse acetic acid softening; 2, OsO_4 fixative concentration from the original 1% increase to 2%; 3, each step dehydration, penetration time extension; 4, with the tablet positioning package