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目的:采用新型反相手性色谱柱Chiralpak AD-RH(直链淀粉3,5-二甲基苯基氨基甲酸酯涂敷在5μm硅胶上),建立萘哌地尔对映体的反相高效液相色谱拆分方法,为萘哌地尔手性对映体药代动力学研究提供基础。方法:在Chiralpak AD-RH柱上,考察了流动相pH、流速、乙腈比例、缓冲盐浓度、柱温对萘哌地尔手性对映体拆分的影响。结果:以20mmol·L-1磷酸盐缓冲液(pH5.7)-乙腈(40∶60,v/v)为流动相,流速0.6mL·min-1,检测波长284nm,柱温30℃,在Chiralpak AD-RH手性柱上成功拆分萘哌地尔对映体。结论:萘哌地尔对映体在Chiralpak AD-RH手性柱上获得基线拆分。
OBJECTIVE: To establish a reversed phase high performance enantiomer of naftopidil enantiomers using a novel reversed phase chiral column Chiralpak AD-RH (amylose 3,5-dimethylphenyl carbamate coated on 5 μm silica gel) Liquid chromatographic resolution method provides the basis for the pharmacokinetics study of chiral enantiomers of naftopidil. Methods: Chiral enantiomers of Naftopidil were separated on a Chiralpak AD-RH column with mobile phase pH, flow rate, acetonitrile ratio, buffer concentration and column temperature. Results: The mobile phase consisted of 20mmol·L-1 phosphate buffer (pH5.7) -acetonitrile (40:60, v / v) with a flow rate of 0.6mL · min-1 and a detection wavelength of 284nm. Enantioseparation of Naftopidil Enantiomers on Chiralpak AD-RH Chiral Column. Conclusion: The enantiomer of naftopidil obtained a baseline resolution on a Chiralpak AD-RH chiral column.