论文部分内容阅读
目的 :探讨载脂蛋白E(ApoE)基因多态性与动脉粥样硬化性脑梗死的关系。方法 :以聚合酶链反应 -限制性片段长度多态性 (PCR -RFLP)技术结合银染色方法检测了 40例动脉粥样硬化性脑梗死 (ACI)患者和 90例无血缘关系的健康汉族人群载脂蛋白E基因型。结果 :ACI组ε4 等位基因频率明显高于对照组 (P <0 .0 1) ,ε3等位基因频率明显低于对照组 (P <0 .0 1)。结论 :ε4 等位基因是动脉粥样硬化性脑梗死的遗传易患因子之一 ,ε3等位基因是动脉粥样硬化性脑梗死的保护因子。
Objective: To investigate the relationship between apolipoprotein E (ApoE) gene polymorphism and atherosclerotic cerebral infarction. Methods: Forty patients with atherosclerotic cerebral infarction (ACI) and 90 unrelated Han healthy people were detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) combined with silver staining. Apolipoprotein E genotype. Results: The frequency of ε4 allele in ACI group was significantly higher than that in control group (P <0.01). The frequency of ε3 allele in ACI group was significantly lower than that in control group (P <0.01). Conclusion: The ε4 allele is one of the genetic predisposing factors in atherosclerotic cerebral infarction. The ε3 allele is a protective factor in atherosclerotic cerebral infarction.