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目的:探讨健脾益肠散对实验性溃疡性结肠炎(UC)大鼠细胞间黏附分子1(ICAM-1)的影响。方法:将健康SPF级雄性SD大鼠60只,随机分为正常组和造模组;造模组采用TNBS/乙醇法复制UC大鼠模型;待复制模型成功后将造模组随机分为5组,分别为模型组、西药组及中药高、中、低剂量组,每组10只;灌胃相应药物3周后,HE染色检测各组大鼠结肠黏膜组织损伤情况,同时采用ELISA法检测血清中ICAM-1的水平,免疫组化法检测结肠组织中ICAM-1的表达。结果:模型组与正常组相比,大鼠结肠黏膜损伤评分、血清ICAM-1水平及结肠组织中ICAM-1表达均明显升高(P<0.01)。中药高、中剂量组血清ICAM-1水平和高、中、低剂量组结肠黏膜损伤评分、ICAM-1表达均较模型组下降(P<0.05或P<0.01)。中药中剂量组对ICAM-1的影响与西药组比较,差异无统计学意义(P>0.05)。结论:健脾益肠散可能通过调节ICAM-1而达到对UC大鼠肠黏膜的免疫保护,从而发挥治疗作用。
Objective: To investigate the effect of Jianpi Yichang San on intercellular adhesion molecule-1 (ICAM-1) in experimental ulcerative colitis (UC) rats. METHODS: Sixty healthy SPF male Sprague Dawley rats were randomly divided into normal group and modeling group. UCBS model was established by TNBS/ethanol method. The model group was randomly divided into 5 groups after the replication model was successful. The groups were model group, western medicine group and traditional Chinese medicine high, middle and low dose groups, 10 in each group. After 3 weeks of gavage of corresponding drugs, HE staining was used to detect the damage of colon mucosal tissues in each group and detected by ELISA. Serum ICAM-1 levels were measured by immunohistochemistry for ICAM-1 expression in colon tissues. RESULTS: Compared with the normal group, the rats in the model group had significantly increased colonic mucosal injury scores, serum ICAM-1 levels, and ICAM-1 expression in colon tissues (P<0.01). The scores of serum ICAM-1 in the high and middle doses of traditional Chinese medicine and the high, middle and low doses of the colon mucosal injury score and ICAM-1 expression were lower than those in the model group (P<0.05 or P<0.01). There was no significant difference in the effect of medium dose group on ICAM-1 compared with western medicine group (P>0.05). Conclusion: Jianpiyichangsan may reach the intestinal mucosa of UC rats by regulating ICAM-1 to achieve its therapeutic effect.