M852-1是由柔软滨麦草和普通小麦7182经杂交和回交培育的易位系。苗期抗病性鉴定结果表明,M852-1对CYR29、CYR31、CYR32、CYR33、Su11-4、Su11-7和V26等7个中国小麦条锈菌主要生理小种或新的致病类型均表现免疫至高抗,是一个较好的抗条锈资源材料。用条锈菌流行小种CYR33对M852-1与铭贤169杂交F1、F2、F3和BC1代进行抗性鉴定与遗传分析,发现M852-1对CYR33的抗条锈性由1对隐性基因控制,暂定名为Yr Elm。以F2代分离群体构建作图群体,利用集群分离分析法,筛选到与Yr Elm连锁的5个SSR标记:Xcfd35、Xgwm161、Xwmc630、Xgwm533和Xcfd34,并将Yr Elm定位于小麦染色体3DS上。Yr Elm两侧最近2个SSR标记Xcfd35与Xgwm161的遗传距离分别为6.5 c M和4.2 c M。抗锈性鉴定、系谱分析以及分子标记检测结果表明,该抗病基因来源于柔软滨麦草。综合基因来源、分子检测及染色体位点等方面的分析,认为Yr Elm可能是一个新的抗条锈病基因。用该基因两侧最近两个标记Xcfd35和Xgwm161检测68个甘肃和黄淮麦区小麦品种(系),10个(14.7%)品种能扩增出与M852-1相同的条带。进一步进行抗病性及系谱分析表明,这10个品种均不含Yr Elm。本研究结果为利用Yr Elm进行分子标记辅助育种和进一步的精细定位奠定了基础。 M852-1 is a translocation line that is bred and backcrossed by Sophora alopecurosum and common wheat 7182. The results of seedling disease resistance showed that M852-1 showed resistance to 7 major pathogenic strains or new pathogenicity strains of Chinese wheat stripe rust, such as CYR29, CYR31, CYR32, CYR33, Su11-4, Su11-7 and V26 Immune to high resistance, is a better resistance to stripe rust resources. Resistance identification and genetic analysis of F1, F2, F3 and BC1 generations of M852-1 and Mingxian 169 were carried out with CYR33, a strain of stripe rust resistance. The resistance of stripe rust of M852-1 to CYR33 was determined by one pair of recessive genes Control, tentatively named Yr Elm. Five SSR markers linked to Yr Elm, Xcfd35, Xgwm161, Xwmc630, Xgwm533 and Xcfd34, were screened by cluster segregation analysis in F2 segregating population. Yr Elm was mapped on wheat chromosome 3DS. The genetic distances of Xcfd35 and Xgwm161 from the two most recent SSR markers on both sides of Yr Elm were 6.5 cM and 4.2 cM, respectively. The results of rust resistance identification, pedigree analysis and molecular marker showed that the resistance gene originated from Sophora alopecuroids. Based on the analysis of source, molecular detection and chromosomal location, Yr Elm may be a new stripe rust resistance gene. Sixty-eight Gansu and Huanghuai wheat wheat cultivars (lines) were detected with the two most recent markers Xcfd35 and Xgwm161 on both sides of the gene. The same bands as M852-1 were amplified in 10 (14.7%) varieties. Further resistance and pedigree analysis showed that none of the 10 cultivars contained Yr Elm. The results of this study laid the foundation for Yr Elm molecular marker-assisted breeding and further fine mapping.