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目的了解广西食源性致病菌监测结果的准确性,评价广西食源性致病菌监测网络实验室检验技术水平,探讨提高食源性致病菌监测网络实验室检验技术水平的方法。方法分别依据2011年、2012年各年度《食源性致病菌监测工作手册》)进行菌株鉴定。结果 2011年-2012年广西各市、县网络实验室上送食源性致病菌分离菌株11类共1649株,经鉴定符合菌株1408株,符合率85.39%。创伤弧菌鉴定符合率最高(96.77%),出血性大肠埃希氏菌最低(42.86%);11类菌株中有4类(36.36%)鉴定符合率低于80%。2011年、2012年上送菌株鉴定符合率分别为87.03%、84.19%,二者比较差异无统计学意义(χ2=2.60,P>0.05);市级、县级网络实验室上送菌株鉴定符合率分别为87.92%、82.61%,二者比较差异有统计学意义(χ2=9.29,P<0.05)。结论广西市、县食源性致病菌监测网络实验室检测能力及技术水平还比较低,应加大实验室能力建设的投入,不断提高实验室的检验技术水平。
Objective To understand the accuracy of monitoring results of food borne pathogens in Guangxi and evaluate the laboratory techniques of foodborne pathogens monitoring in Guangxi and to explore ways to improve the laboratory techniques of foodborne pathogenic bacteria monitoring networks. Methods The strains were identified according to the “Foodborne Pathogen Monitoring Manual” in 2011 and 2012, respectively. Results From 2011 to 2012, 1649 strains of 11 strains of food-borne pathogenic bacteria were obtained from network labs of cities and counties in Guangxi, and 1408 strains were identified. The coincidence rate was 85.39%. Vibrio vulnificus was the highest (96.77%) and hemorrhagic Escherichia coli was the lowest (42.86%). Four out of 11 strains (36.36%) were identified as less than 80%. The coincidence rates of the sent strains in 2011 and 2012 were 87.03% and 84.19%, respectively. There was no significant difference between them (χ2 = 2.60, P> 0.05) The rates were 87.92% and 82.61% respectively, with significant difference between them (χ2 = 9.29, P <0.05). Conclusion The detection ability and technical level of foodborne pathogenic bacteria monitoring network laboratory in Guangxi are relatively low. The investment in capacity building of the laboratory should be increased to continuously improve the laboratory testing technology.