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为观察豚鼠暴露于强噪声后畸变产物耳声发射(DPOAE)及神经元特异性烯醇化酶(NSE)的变化,选用13只Preyer’s反射正常的健康豚鼠,分为二组,8只噪声暴露组,5只为NSE表达对照组。噪声强度115dB(A),连续暴露4小时,DPOAE幅值于噪声暴露前后进行测试,结果DPOAE幅值噪声暴露前后差异明显(P<0.001),豚鼠内耳内、外毛细胞及螺旋神经细胞胞浆、隧道贯穿纤维NSE免疫组化反应均呈阳性表达,暴震前后无明显变化。结果提示豚鼠接受短时间强噪声刺激后,DPOAE幅值的下降为暂时阈移,而内耳神经元及其末梢未受损伤。
To observe the changes of distortion product otoacoustic emissions (DPOAE) and neuron-specific enolase (NSE) in guinea pigs exposed to strong noises, 13 healthy healthy guinea pigs with normal Preyer’s reflexes were selected and divided into two groups. Eight noise exposed groups , 5 were NSE expression control group. The amplitude of DPOAE was measured before and after noise exposure at 115dB (A) and 4 hours of continuous exposure. The results showed that there was significant difference (P <0.001) before and after DPOAE amplitude noise exposure. The internal and external hair cells and the cytoplasm of spiral nerve cells , NSE immunohistochemical staining of the tunnel through the fiber were positive expression, no significant changes before and after the storm shock. The results suggest that guinea pigs receiving short-term strong noise stimulation, DPOAE amplitude decreased temporarily for the threshold, while the inner ear neurons and their terminals are not damaged.