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目的研究内吗啡肽对泻剂结肠大鼠结肠肌电活动的影响,探讨慢传输性便秘的发病机制。方法建立泻剂结肠动物模型,测定内吗啡肽1和内吗啡肽2对大鼠结肠肌电活动的影响。结果泻剂组大鼠结肠慢波频率和振幅分别为(28.19±7.51)次/min和(0.076±0.018)mV,与对照组比较[(36.05±8.94次/min)和(0.600±0.310)mV]明显降低;内吗啡肽1、内吗啡肽2以浓度依赖方式抑制泻剂结肠的慢波肌电活动,振幅明显降低,频率[(2 8.18±7.51)次/min3无明显变化,内吗啡肽1的作用强于内吗啡肽2。注射内吗啡肽不能阻断乙酰胆碱对结肠的兴奋作用。阿片受体拮抗剂纳洛酮(naloxone)能逆转内吗啡肽的抑制作用。结论内吗啡肽参与了泻剂结肠大鼠结肠肌电活动和结肠动力的调控,可能是慢传输性便秘发病的重要因素之一。
Objective To study the effect of endomorphin on colonic myoelectric activity in colonic laxative colon and to explore the pathogenesis of slow transit constipation. Methods The model of laxative colon was established to determine the effect of endomorphin-1 and endomorphin-2 on colonic myoelectric activity in rats. Results The frequency and amplitude of slow wave of colon in colon of the laxative group were (28.19 ± 7.51) and (0.076 ± 0.018) mV, respectively, compared with the control group [(36.05 ± 8.94 and 0.600 ± 0.310) mV ] Significantly decreased; Endorphin-1 and E-morphine 2 inhibited the slow wave EMG activities in a dose-dependent manner in a dose-dependent manner. The amplitude was significantly decreased. The frequency [(2 8.18 ± 7.51) 1 is stronger than endomorphin 2. Injecting endomorphin failed to block the excitatory effect of acetylcholine on the colon. Opioid receptor antagonist naloxone can reverse the inhibitory effect of endomorphin. Conclusions Endomorphin is involved in the regulation of myoelectric activity and colonic motility in colonic laxative colon, which may be one of the important factors in the pathogenesis of slow transit constipation.