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目的:研究塞来昔布联合X线在体外环境下对人胆管癌细胞株QBC939凋亡的影响并对其机制进行初步探讨。方法:CCK-8检测出不同浓度的塞来昔布及不同剂量的X线对QBC939细胞株的增值抑制率,确定塞来昔布组的IC50及X线组的IC50。将QBC939细胞株分为5组:对照组(control)、X线组(R)、塞来昔布组(C)、塞来昔布再加X线组(C+R),X线再加塞来昔布组(R+C)。用流式细胞仪检测各组的凋亡率,western blot检测凋亡相关基因survivin蛋白的表达。结果:联合使用塞来昔布和放疗组的凋亡率有明显的增加,从8.268%,11.233%到15.733%,22.133%(P<0.05)。western结果显示联合组survivin蛋白的表达也明显低于对照组(P<0.05)。先用塞来昔布再加放疗的效果要优于先用放疗后用塞来昔布的效果。结论:塞来昔布联合X线对QBC939细胞株有凋亡增敏作用,作用可能机制之一是通过降低或下调凋亡相关基因survivin蛋白的表达。
Objective: To study the effect of celecoxib combined with X-ray on the apoptosis of human cholangiocarcinoma cell line QBC939 in vitro and its mechanism. Methods: CCK-8 detected different concentrations of celecoxib and different doses of X-ray on QBC939 cell line proliferation inhibition rate to determine the IC50 of celecoxib group and IC50 of X-ray group. The QBC939 cell line was divided into 5 groups: control group, X group (R), celecoxib group (C), celecoxib plus X group (C + R) Coxib group (R + C). The apoptosis rate of each group was detected by flow cytometry, and the expression of survivin protein was detected by western blot. Results: The apoptosis rates of celecoxib group and radiotherapy group were significantly increased from 8.268%, 11.233% to 15.733% and 22.133%, respectively (P <0.05). Western results showed that the expression of survivin protein in the combined group was also significantly lower than that in the control group (P <0.05). Celecoxib combined with radiotherapy is better than the first with celecoxib after radiotherapy. Conclusion: Celecoxib combined with X-ray can enhance the apoptosis of QBC939 cell line. One of the possible mechanisms may be that the expression of survivin protein is down-regulated or down-regulated.