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为探讨淡色库蚊溴氰菊酯抗性的生理生化机理,对抗性和敏感品系进行了离体解毒酶测定、活体抑制试验和抗击倒频率测定。结果:抗性品系的多功能氧化酶和羧酸酯酶活力分别为敏感品系的1.64倍和1.58倍;增效醚(PB)、磷酸三苯酯(TPP)和杀虫脒的增效比分别为152.5、1.1和4.7倍;抗性品系的抗击倒频率高于敏感品系。表明:抗性的主要机理为多功能氧化酶活力增强,其次为靶标不敏感以及羧酸酯酶活力增强
In order to investigate the physiological and biochemical mechanism of deltamethrin resistance in Culex pipiens pallens, the in vitro detoxification enzyme assay, in vivo inhibition test and anti-knockdown frequency determination were performed on the resistant and susceptible lines. Results: The activities of multifunctional oxidase and carboxylesterase of the resistant lines were 1.64 and 1.58 times higher than those of the susceptible lines respectively. The concentrations of PB, TPP and chlordimeform The synergistic ratios were 152.5, 1.1 and 4.7 times, respectively. The resistance frequency of the resistant lines was higher than that of the susceptible lines. The results showed that the main mechanism of resistance was multifunctional oxidase activity enhancement, secondly target insensitivity and carboxylesterase activity enhancement