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目的:优选川芎和细辛挥发油的β-环糊精(β-CD)微囊萃取工艺条件。方法:选择β-CD和硫酸钠作为双水相萃取剂,以挥发油收率、甲基丁香酚和苯酞类物质(以藁本内酯计)质量的综合评分为指标,通过正交试验考察萃取液中β-环糊精和硫酸钠的质量浓度、提取方式、萃取温度对微囊萃取工艺的影响。采用GC-MS分析挥发油的组分,以邻二甲苯和邻苯二甲酸二丁酯为内标物。结果:最佳萃取工艺条件为加3倍量40%乙醇室温下浸泡24 h,60℃热浸60 min,抽滤,提取液中加入β-CD和硫酸钠至质量浓度分别为300,50 g·L-1,50℃振荡萃取30 min;挥发油收率86.54%,挥发油中甲基丁香酚和苯酞类物质(以藁本内酯计)质量分别为75.88,353.91 mg。结论:β-CD微囊双水相萃取及包合川芎和细辛挥发油的工艺合理可行,适合于采用β-CD包合的挥发油制剂工业化生产。
Objective: To optimize the extraction conditions of β-cyclodextrin (β-CD) microcapsules of Chuanxiong and Asarum essential oil. Methods: β-CD and sodium sulfate were chosen as the extractants of aqueous two-phase system. The yield of volatile oil, the total score of the quality of methyl eugenol and phthalide (based on ligustilide) Effect of β - Cyclodextrin and Sodium Sulfate Concentration, Extraction Methods and Extraction Temperature on Extraction Process of Microcapsules. GC-MS analysis of volatile oil components, o-xylene and dibutyl phthalate as the internal standard. Results: The optimal extraction conditions were as follows: adding 3 times the amount of 40% ethanol for 24 h at room temperature and hot-dipping at 60 ° C for 60 min. Filtering, the β-CD and sodium sulfate were added to the extract to give mass concentrations of 300 and 50 g L-1, 50 ℃ for 30 min. The yield of volatile oil was 86.54%. The mass of methyl eugenol and phthalide in the volatile oil were 75.88 and 353.91 mg, respectively. Conclusion: The extraction of β-CD microcapsules in aqueous phase and the inclusion of Ligusticum chuanxiong and volatile oil of Volatile Oil are reasonable and feasible, which are suitable for the industrialized production of volatile oil preparations with β-CD inclusion.