目的:建立HPLC法测定氯柳硼酊中氯霉素和水杨酸含量的方法。方法:色谱柱Zorbax ODS-C_(18)(150 mm×4.6 mm,5μm);以甲醇-2%乙酸溶液(48:52v/v)为流动相;流速1.0 ml·min~(-1);检测波长为301 nm,柱温30℃。结果:氯霉素在10～60μg·ml~(-1)范围内,浓度与峰面积线性关系良好(r=0.9995),平均回收率99.5%,RSD=0.7%;水杨酸在10～60μg·ml~(-1)范围内,浓度与峰面积线性关系良好(r=0.9995),平均回收率100.2%,RSD=0.6%。结论:该方法专属性好,操作简便、快速,结果准确,可用于氯柳硼酊制剂的质量控制。 Objective: To establish a method for the determination of chloramphenicol and salicylic acid in clotrimazole tincture by HPLC. METHODS: The mobile phase consisted of Zorbax ODS-C 18 (150 mm × 4.6 mm, 5 μm) and methanol-2% acetic acid solution (48:52 v / v) at a flow rate of 1.0 ml · min -1. The detection wavelength was 301 nm and the column temperature was 30 ℃. Results: The calibration curve of chloramphenicol in the range of 10 ~ 60μg · ml ~ (-1) was linear (r = 0.9995) with the average recovery of 99.5% and RSD = 0.7% · Ml ~ (-1). The linear relationship between concentration and peak area was good (r = 0.9995). The average recovery was 100.2% and the RSD was 0.6%. Conclusion: The method is specific, easy to operate, rapid and accurate. It can be used for the quality control of Borneol Tincture.