小核糖核酸病毒科是最大的致病科之一,它们引起了许多人和动物的严重疾病。为了解该病毒的结构情况,长期以来,许多病毒学家曾作过艰苦的努力,设法澄清它们的三维结构。早在1959年,曾有人用X线晶体衍射的方法研究脊髓灰质炎病毒和柯萨奇病毒,但由于技术上的原因,获得信息很少。后来人们又分别采用化学标记、交联试剂处理以及把病毒与特异性抗体反应等 Picornaviridae are one of the largest causative families and cause serious illness in many people and animals. To understand the structure of the virus, many virologists have long struggled to find ways to clarify their three-dimensional structure. As early as 1959, polio and Coxsackie viruses were studied by X-ray crystallography, but little information was obtained for technical reasons. Later, people also used chemical markers, cross-linking reagents and the reaction of the virus and specific antibodies, respectively