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本研究采用顶果木叶芽作外植体,研究总结了顶果木的组织培养繁殖技术。结果表明,叶芽外植体用70%酒精消毒10s,再用10%的H2O2消毒5min效果最好,叶芽外植体转接到诱导分化培养基后,暗培养7d后再全光培养,诱导成功率达55.2%。顶果木适宜的继代增殖培养基为MS+6-BA0.5mg/L+KT0.5mg/L+NAA0.2mg/L,增殖系数可达3.6。组培芽苗采用生根培养基1/2MS+NAA0.5 mg/L+IBA0.5mg/L+6-BA0.3mg/L培养,生根率可达82%。
In this study, the use of top fruit buds as explants, the study summarized the top wood tissue culture and propagation techniques. The results showed that the explants of leaf buds were disinfected with 70% alcohol for 10s and disinfected with 10% H2O2 for 5min. The explants of leaf buds were transferred to differentiation medium and then cultured for 7 days in dark. Rate of 55.2%. The suitable subculture medium of top fruit is MS + 6-BA0.5mg / L + KT0.5mg / L + NAA0.2mg / L, the multiplication coefficient can reach 3.6. The rooting rate of the tissue culture buds was up to 82% using rooting medium 1 / 2MS + NAA0.5 mg / L + IBA0.5 mg / L + 6-BA0.3 mg / L.