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为探讨重组肿瘤坏死因子－α（ｒＴＮＦ－α）、重组白细胞介素－６（ｒＩＬ－６）与内皮素－１（ＥＴ－１）在肝坏死中的作用；用Ｄ－氨基半乳糖（Ｄ－ＧａｌＮ）单独或联合ｒＴＮＦ－α，ｒＩＬ－６对ＳＤ大鼠进行腹腔注射，常规检测其肝功能及ＥＴ－１含量的变化，并通过病理学检查其肝坏死的程度。结果发现，在Ｄ－ＧａｌＮ造成肝坏死的基础上，合用ｒＴＮＦ－α，ｒＩＬ－６可增加肝坏死的程度，使血清中总胆红素（ＴＢＩＬ）、谷丙转氨酶（ＡＬＴ）及ＥＴ－１均明显升高。提示ｒＩＬ－６，ＥＴ－１在ｒＴＮＦ－α介导的肝细胞损伤中起较大的作用。
To investigate the effect of rTNF-α, rIL-6 and ET-1 on hepatic necrosis in rats, the effects of recombinant human D-galactosamine -GalN) alone or in combination with rTNF-αand rIL-6. The liver function and the content of ET-1 were detected routinely. The extent of hepatic necrosis was examined by pathology. The results showed that the combination of rTNF-αand rIL-6 could increase the degree of hepatic necrosis and the total bilirubin (TBIL), alanine aminotransferase (ALT) and ET-1 Significantly increased. These results suggest that rIL-6 and ET-1 play important roles in rTNF-α-mediated hepatocellular injury.