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蜂斗菜(Petasites Japonicus Fr.Schmidt)是雌雄异株作物,通常只种植雌株,用地下茎繁殖,再加上三倍体栽培种的不孕性(2 n=87,x=29),进行杂交育种极其困难.本文研究了蜂斗菜叶肉原生质体的分离和培养,并讨论了在育种上利用的可能性. 取供试品种“Aichi-Wase-buki”生长的地下茎15～20 cm进行表面消毒,首先插入70%酒精60 S,再浸入有效氯为0.5%的漂白粉溶液10min.消毒后,在解剖镜下剥取带有叶原基的茎尖(0.5～1.1mm)接种于Ms+BA 2 mg/l的培养基上.待茎叶伸出后,切割移植在Ms+BA
Petasites Japonicus Fr.Schmidt is a dioecious plant usually planted with female plants and propagated with rhizomes, plus triploid inoculation (2 n = 87, x = 29) Hybrid breeding is extremely difficult.This paper studied the isolation and culture of the protoplasts of the beetroot mesophyll and discussed the possibility of using it in breeding.The underground stems grown at 15-20 cm for the test variety “Aichi-Wase-buki” Disinfection, the first to insert 70% alcohol 60 S, and then immersed in the available chlorine 0.5% bleach solution 10min.After disinfection, stripped root tips with leaf primordia (0.5 ~ 1.1mm) were inoculated on Ms + BA 2 mg / l medium until the stems and leaves are stretched out, cut and transplanted in Ms + BA