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用高效液相色谱法测定苹果果皮多种类胡萝卜素。以丙酮与石油醚提取色素 ,用NovapakC18(4 .6mm×15 0mm)色谱柱 ,乙腈∶甲醇∶二氯甲烷 (70∶2 0∶10 )流动相 ,在 45 0nm下检测类胡萝卜素。结果表明 ,玉米黄质、叶黄素、番茄红素与 β 胡萝卜素的回收率分别是94.2 7%～ 99.82 %、84.5 6 %～ 89.41%、87.5 3～ 93.5 4%与 88.0 8%～93 .2 4%。其中富士果仅检测到玉米黄质 (5 .9× 10 -3 μg/mm2 )与 β 胡萝卜素 (5 .4× 10 -3 μg/mm2 ) ;蛇果 (红星 )只有玉米黄质 (9.1× 10 -3 μg/mm2 )。
Determination of Various Carotenoids in Apple Peel by High Performance Liquid Chromatography. The pigment was extracted with acetone and petroleum ether, and the carotenoid was detected at 450 nm using a Novapak C18 (4.6 mm × 150 mm) column and a mobile phase of acetonitrile: methanol: dichloromethane (70: 2 0:10). The results showed that the recoveries of zeaxanthin, lutein, lycopene and β-carotene were 94.2 7% -99.82%, 84.5 6% -89.41%, 87.5 3-93.5 4% and 88.0 8% -93, respectively. twenty four%. Fuji fruit only detected zeaxanthin (5.9 × 10-3 μg / mm2) and β-carotene (5.4 × 10-3 μg / mm2); only snail yellow (9.1 × 10 -3 μg / mm 2).