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【目的】探讨基质金属蛋白酶9(MMP9)在大肠癌组织中的表达及其与大肠癌侵袭转移关系,基底膜(BM)的状态与大肠癌发生发展的关系。【方法】采用链霉卵白素过氧化物酶(SP法)免疫组织化学方法检测51例大肠癌1、5例肠腺瘤1、0例正常肠粘膜MMP9的表达与Ⅳ型胶原蛋白的表达情况。【结果】51例大肠癌MMP9阳性率84.3%,明显高于正常肠粘膜(20.0%)和肠腺瘤(26.7%)(P<0.05和P<0.01);MMP9的表达与大肠癌的分化程度、淋巴结转移及Dukes分期密切相关(P<0.05或P<0.01);正常肠粘膜和肠腺瘤组织中,基底膜Ⅳ型胶原连续完整,大肠癌基底膜Ⅳ型胶原分布间隔缺损者明显高于肠粘膜和肠腺瘤(P<0.01)。【结论】MMP9对肠癌基底膜破坏起着十分重要作用,且可作为预测大肠癌侵袭、转移的重要指标。
【Objective】 To investigate the expression of matrix metalloproteinase 9 (MMP9) in colorectal carcinoma and its relationship with the invasion and metastasis of colorectal carcinoma and the relationship between the status of basement membrane (BM) and the occurrence and development of colorectal carcinoma. 【Methods】 The expression of type Ⅳ collagen and MMP9 in 51 cases of colorectal carcinoma and 1 case of enteric adenoma in 1 case and 1 case of normal intestinal mucosa were detected by streptavidin peroxidase (SP method) immunohistochemistry . 【Results】 The positive rate of MMP9 in 51 cases of colorectal carcinoma was 84.3%, which was significantly higher than that of normal intestinal mucosa (20.0%) and adenomas (26.7%) (P <0.05 and P <0.01) , Lymph node metastasis and Dukes stage (P <0.05 or P <0.01). In normal intestinal mucosa and intestinal adenoma tissues, the type Ⅳ collagen of basement membrane was continuous and complete. The type Ⅳ collagen distributed gap in basement membrane was significantly higher than that in colorectal carcinoma Intestinal mucosa and enteric adenoma (P <0.01). 【Conclusion】 MMP9 plays an important role in the destruction of the basement membrane of colorectal cancer, and may be used as an important index to predict the invasion and metastasis of colorectal cancer.