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目的检测支气管哮喘患者外周血CD4+T细胞、CD4+ CD25+T细胞、CD4+ FOXP3+T细胞三类亚群,分析其在支气管哮喘患者的表达。方法选择112例支气管哮喘患者和56例正常人作为对照,采用流式细胞术检侧患者外周血的CD4+T细胞、CD4+ CD25+ T细胞、CD4+ FOXP3+T细胞。结果 FOXP3主要表达在CD4+ CD25+T细胞上,CD4+ CD25+T细胞、CD4+FOXP3+T细胞与对照组相比均明显降低(P<0.01),急性发作期哮喘患者外周血中CD4+ CD25+ T细胞、CD4+ FOXP3+T细胞明显低于非急性发作期(P<0.01),与疾病的活动性相关。结论支气管哮喘患者存在明显的免疫功能失调,CD4+ CD25+T细胞、CD4+ FOXP3+ T细胞在疾病活动中起着重要作用,是导致患者细胞免疫功能失调的重要原因。
Objective To detect the levels of CD4 + T cells, CD4 + CD25 + T cells and CD4 + FOXP3 + T cells in peripheral blood of patients with bronchial asthma and analyze their expression in patients with bronchial asthma. Methods One hundred and twelve patients with bronchial asthma and 56 normal controls were selected as control. CD4 + T cells, CD4 + CD25 + T cells and CD4 + FOXP3 + T cells in peripheral blood were detected by flow cytometry. Results FOXP3 was mainly expressed on CD4 + CD25 + T cells, CD4 + CD25 + T cells and CD4 + FOXP3 + T cells were significantly decreased compared with the control group (P <0.01). CD4 + CD25 + T cells in peripheral blood of patients with acute exacerbation , CD4 + FOXP3 + T cells were significantly lower than non-acute exacerbation (P <0.01), and the activity of the disease. Conclusions There are obvious immune dysfunction in patients with bronchial asthma. CD4 + CD25 + T cells and CD4 + FOXP3 + T cells play an important role in the disease activity, which is the important reason leading to cellular immune dysfunction.