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目的 探讨青、中、老年人大肠癌临床病理特点与 6种细胞周期蛋白表达的关系。方法 采用SP免疫组化法对 89例青、中、老年人大肠癌进行 6种细胞周期蛋白检测。结果 cyclinD1,mdm2 ,p5 3 ,p2 1(waf1/cip1) ,cdk4,p16在各年龄组均见表达 ,青年组大肠癌组织学分级Ⅲ级、弥漫浸润型的病例及中年组临床C期病例明显高于老年组 (P <0 .0 5 ) ,老年组临床B期病例明显高于青年组 (P <0 .0 5 ) ,周期蛋白表达p5 3青年组明显低于中老年组 ,p2 1青、中年组明显低于老年组 (P <0 .0 5 )。结论 中、老年人大肠癌细胞周期蛋白以p5 3调控点改变为主 ,青、中年人大肠癌的临床病理特点可能与p2 1,CyclinD1表达有关。
Objective To investigate the relationship between the clinicopathological features of colorectal cancer, the expression of six cyclins, and the expression of cyclin. Methods Immunohistochemical SP method was used to detect six kinds of cell cycle proteins in 39 cases of colorectal cancer in the middle, middle and old ages. RESULTS: CyclinD1, mdm2, p53, p21 (waf1/cip1), cdk4, p16 were all expressed in all age groups. The young group had histological grade III, diffuse infiltrative, and middle-aged clinical C stage cases. Significantly higher than the elderly group (P < 0.05), the clinical B stage cases in the elderly group were significantly higher than the young group (P <0.05), and the cyclin expression p53 group was significantly lower in the young group than in the middle-aged group, p2 1 The young and middle-aged groups were significantly lower than the old-aged group (P < 0.05). Conclusions The cyclin of colorectal cancer in middle-aged and elderly patients is mainly altered by the p53 regulatory site. The clinicopathological features of colorectal cancer in young and middle-aged people may be related to the expression of p21 and CyclinD1.